Feedback regulation of ribosomal protein gene expression in Escherichia coli: structural homology of ribosomal RNA and ribosomal protein MRNA.
نویسندگان
چکیده
منابع مشابه
Feedback regulation of ribosomal protein gene expression in Escherichia coli: structural homology of ribosomal RNA and ribosomal protein MRNA.
Certain ribosomal proteins (r proteins) in Escherichia coli, such as S4 and S7, function as feedback repressors in the regulation of r-protein synthesis. These proteins inhibit the translation of their own mRNA. The repressor r proteins so far identified are also known to bind specifically to rRNA at an initial stage in ribosome assembly. We have found structural homology between the S7 binding...
متن کاملFeedback regulation of ribosomal protein gene expression in Escherichia coli.
The structural genes for Escherichia coli ribosomal protein (r-protein) genes L1, S4, and S11 were inserted into a plasmid vector containing the lac operator and promoter such that the synthesis of L1, S4, and S11 was controlled by lac regulatory elements. Synthesis of L1, S4, and S11 was stimulated by addition of an inducer of the lac operon (isopropyl thiogalactoside) to exponentially growing...
متن کاملTranslational regulation by ribosomal protein S8 in Escherichia coli: structural homology between rRNA binding site and feedback target on mRNA.
It has been previously shown that ribosomal protein synthesis in Escherichia coli is regulated at the level of translation by certain key ribosomal proteins. In the spc operon, S8 regulates the expression of L5 and some of the subsequent genes, while the first two genes (L14 and L24) are regulated independently. We therefore determined the DNA sequence at the junction of the L24 and L5 genes, w...
متن کاملStringent control of ribosomal protein gene expression in Escherichia coli.
The regulation of the expression of ribosomal protein genes was examined during partial inhibition of tRNA aminoacylation in isogenic rel(+) and rel(-) strains of E. coli B carrying a temperature-sensitive mutation in valyl-tRNA synthetase (EC 6.1.1.9) gene. Measurements of the differential synthesis rate of ribosomal protein, alpha(r) (ribosomal protein synthesis rate/total protein synthesis r...
متن کاملRegulation of ribosomal protein synthesis in Escherichia coli.
The kinetics of synthesis of ribosomal, nonribosomal, and total protein, and of ribosomal ribonucleic acid (RNA), were measured in Escherichia coli during a shift-up involving a doubling of specific growth rate. The increase in ribosomal protein synthesis was not accompanied by a corresponding decrease in non-ribosomal protein synthesis. Thus, the average rate of protein synthesis per ribosome ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1980
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.77.12.7084